P-245: Vitamin D Receptor FOKI and BSMI Polymorphisms in Women with Recurrent Abortions in Iranian Population

Background: Recurrent spontaneous abortion (RSA) is a frequent reproductive problem, with three or more abortions affecting 1 to 2%, two or more abortions affecting up to 5% of women of reproductive age. Some polymorphisms are supposed to be etiology in this group.Vitamin D receptor (VDR) gene polymorphism seems to be a good ones to be searched due to their function on placenta. Materials and Methods: 150 women with idiopathic RSA who had history of two or more successive spontaneous abortions were suffering from recurrent abortion who referred to Avicenna Infertility clinic and 100 healthy controls with at least two live children. Blood samples were used to extract DNA, by using the salting out method from peripheral blood samples and then PCR-RFLP designed for BSM1 and FOKI VDR gene. The results were analyzed using Chi Square Test while a p<0.05 was considered to be significant. Results: The frequencies of (VDR) BSMI BB, Bb and bb genotypes were 18.6% (47), 60.9% (154), and 20.6% (52), respectively, in the sample. The frequencies of BB, Bb, and bb genotypes were 13.0% (13), 64.0% (64), and 23.2% (23), respectively, in control group and 22.2 % (34),58.8% (90), and 19% (29), respectively, in RSA patients. The proportion of BSMI demonstrated no significant differences between RSA patients and healthy subjects (p=0.171). In contrast, while FF homozygote was the largest group in FOKI genotype, FF normal, Ff heterozygote and ff homozygote are (50) 60.2% and (23) 60.3 %, (7) 8.4% respectively in control group and (84) 54%,( 50) 32.5%.(20) 13% respectively in RSA patients. The proportion of FOKI demonstrated no significant difference between RSA patients and healthy subjects (p=0.278). Conclusion: In the present study, there was no significant difference between case and control group in an Iranian population of reproductive age women.